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KMID : 0525720130180010007
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Journal of Chitin and Chitosan
2013 Volume.18 No. 1 p.7 ~ p.12
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Effect of Molecular Weight of Chitosan on Microbial Growth and γ-cyclodextrin Production from Bacillus cereus GU-02
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Goo Bon-Geun
Shin Da-Eun
Choi Na-Eun
Park Jae-Kweon
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Abstract
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Effects of chitosan with different molecular weight on gamma-cyclodextrin (γ-CD) production and bacterial cell growth of Bacillus cereus GU-2 was elucidated. Optimum pH and temperature for bacterial growth and γ-CD production were estimated to be pH 8~10 and 37 o C, respectively. Interestingly, among bioactive substances chitosan showed molecular weight dependentinhibitory effect (over 80%) on growth of B. cereus GU-2. Among, MMWC-2 (3,500 Da) revealed the highest (92%) inhibitory effect on bacterial cell growth, considerable as a limiting factor. Consequently, no significant inhibitory effect of both high molecular weight chitosan (HMWC, 200 kDa) and MMWCs on the production of γ-CD was observed. These results made us to hypothesize that γ-CD synthesized by B. cereus GU-2 may be a key modulator involved in bacterial cell viability.
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KEYWORD
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Chitosan, Molecular weight, Bacillus sp., Gamma-cyclodextrin, γ-CD, Antibacterial activity
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